Recovery ⏱ Half-life: ~2-3 hours (estimated)

Thymosin Beta-4

Thymosin Beta-4 (Tβ4) — Full 43-amino acid Protein

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Half-Life
~2-3 hours (estimated)
Purity
≥98%
Mol. Weight
4,921 Da
Form
Lyophilized powder

What is Thymosin Beta-4?

Thymosin Beta-4 is the full-length 43-amino acid protein from which the popular research peptide TB-500 is derived. While TB-500 fragments capture some of Tβ4’s activity, the full molecule has additional functions related to actin sequestration, gene regulation, and stem cell biology. It is one of the most extensively studied regenerative proteins in preclinical and early clinical development.

Dosage Information (Research Use)

Animal research: 6-150 μg per dose depending on model. Topical (corneal): 0.01-0.1% solution. Clinical trials used various routes. Investigational — not commercially available as a therapeutic.

Reconstitution & Handling

Reconstitute lyophilized powder in sterile water or saline.

Half-Life & Pharmacokinetics

~2-3 hours (estimated)

Reported Observations in Literature

Generally well tolerated in clinical trials. Topical formulations show minimal adverse events. Systemic administration safety profile similar to TB-500. Theoretical concern about promoting growth of existing cancers (angiogenesis).

Key Research References

  • Goldstein AL et al. “Thymosin β4: a multi-functional regenerative peptide.” Expert Opin Biol Ther. 2012;12:37-51
  • Dunn SP et al. “Treatment of chronic nonhealing neurotrophic corneal epithelial defects with thymosin β4.” Ann N Y Acad Sci. 2010;1194:199-206

How Thymosin Beta-4 Works

Thymosin Beta-4 is the full-length protein that TB-500 is derived from. It sequesters actin monomers (G-actin), regulating cytoskeletal dynamics essential for cell migration. The N-terminal tetrapeptide Ac-SDKP (thymosin beta-4 fragment) has distinct anti-fibrotic properties. Tβ4 promotes angiogenesis through VEGF upregulation, stimulates stem cell mobilization, and reduces inflammation through multiple pathways including NF-κB modulation.

Research Applications

🔬 Wound healing and tissue repair
🔬 Cardiac regeneration post-MI
🔬 Corneal healing and dry eye

Research Findings

Phase 2 clinical trials for corneal wound healing (RGN-259 eye drops) showed accelerated epithelial healing. The cardiac repair program demonstrated improved ejection fraction post-MI in animal models. The protein is naturally abundant — the 4th most common transcript in the human body, expressed in all nucleated cells.

Dosage & Administration

Animal research: 6-150 μg per dose depending on model. Topical (corneal): 0.01-0.1% solution. Clinical trials used various routes. Investigational — not commercially available as a therapeutic.

Safety & Side Effects

Generally well tolerated in clinical trials. Topical formulations show minimal adverse events. Systemic administration safety profile similar to TB-500. Theoretical concern about promoting growth of existing cancers (angiogenesis).

Important: All safety information is derived from published research, primarily animal studies. No controlled human clinical trial data exists unless explicitly noted. This compound is sold for research purposes only.

Quick Facts

Molecular Weight 4,921 Da
Half-Life ~2-3 hours (estimated)
Purity ≥98%
Form Lyophilized powder
Storage Lyophilized: -20°C. Reconstituted: 2-8°C.

Key Research References

  • Goldstein AL et al. "Thymosin β4: a multi-functional regenerative peptide." Expert Opin Biol Ther. 2012;12:37-51
  • Dunn SP et al. "Treatment of chronic nonhealing neurotrophic corneal epithelial defects with thymosin β4." Ann N Y Acad Sci. 2010;1194:199-206

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